Table 4 Preference for amino acids at the beginning of highly expressed proteins compared to all proteins datasets. (H₀: there is no difference of amino acid frequencies between all proteins and highly expressed proteins).

E. coli

Ala

3.5E-06

26/9

Lys

0.004

23/11

Ile

7.2E-04

23/9

Lys

3.8E-05

21/7

B. subtilis

Ala

3.6E-06

28/7

-

Gly

0.003

13/3

-

H. pylori

Ala

1.0E-04

26/8

-

-

-

H. influenzae

Ala

3.0E-06

29/9

-

-

Val

0.008

13/5

Ser

0.007

23/11

M. tuberculosis

Ala

6.4E-09

43/12

Lys

1.3E-04

15/3

Tyr

0.005

8/2

Lys

2.5E-04

13/3

Lys

0.003

11/3

Thr

0.007

16/6

T. pallidum

Ala

3.7E-05

29/8

-

-

-

R. prowazekii

Ala

7.6E-04

18/5

Thr

0.003

18/5

-

-

Ser

0.007

27/11

M. genitalium

-

-

-

-

B. burgdorferi

Ala

3.4E-04

19/4

-

-

-

Gly

3.4E-04

15/3

A. fulgidus

Ala

8.6E-06

26/7

-

-

-

M. jannaschii

Ala

1.8E-07

23/4

-

Arg

0.009

13/4

-

P. horikoshii

Ala

0.007

13/4

-

Met

0.004

27/12

-

S. cerevisiae

Ala

3.5E-07

28/8

Arg

1.0E-05

21/6

Val

1.0E-04

18/5

Lys

0.002

20/9

Gly

0.002

14/5

Leu

0.002

0/9

S. pombe

Ala

6.4E-05

28/10

-

-

-

Gly

6.3E-04

17/5

P. falciparum

Ala

1.2E-05

23/5

-

Gln

0.008

11/3

-

Asn

0.003

0/12