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Table 3 Summary of qRT-PCR analysis of the selected genes from the C-AD library

From: Novel subtractive transcription-based amplification of mRNA (STAR) method and its application in search of rare and differentially expressed genes in AD brains

Name

Acc #

Gene Description

% Down

Abundance relative to β-actin*

 

AF498312

integrin-linked kinase1 (ILK)

29%

19.00%

ANK2

NM_020977

ankyrin2, transcript varient 2

no change

11.00%

GABRB3

CR749803

Gamma-aminobutyric acid A receptor, beta 3

no change

98.00%

UBE2B

NM_003337

ubiquitin-conjugating enzyme E2B

45%

33.00%

 

AY339422

NADH dehydrogenase sububit 3

30%

475.00%

TPI1

BC017917

triosephosphate isomerase

41%

208.00%

BCAS1

NM_003657

breast carcinoma sequence 1

20%

71.00%

E46L

AK095309

Ataxin-10

no change

58.00%

SCA1

NM_000332

spinocerebellar ataxia 1

68%

3.00%

DEAF1

BC053322

transcriptional regulator protein, suppressin

24%

7.00%

HLF

M95585

hepatic leukemia factor(HLF)

no change

0.70%

CKIP-1

BC010149

TNF intracellular domain-interacting protein

35%

2.00%

SNCA

NM_000345

synuclein, alpha SNCA

68%

0.20%

CRI1

AL357456

CREBP/EP300 inhibitory protein 1or RBP21

19%

170.00%

SYT4

BC036538

synaptotagmin IV

58%

74.60%

ARF6

BC030291

ADP-ribosylation factor 6, Arp6

59%

0.82%

PRKCE

BC054052

protein kinase C, epsilonY

15%

16.90%

 

BC000143

ELMO2 engulfment and cell motility 2

55%

0.07%

SV2A

BC045111

synaptic vesicle glycoprotein 2A

19%

2.00%

RELN

NM_005045

reelin

82%

1.20%

 

BC042904

hypothetical protein DKFZp564A176

15%

22.20%

 

NM_152722

hypothetical protein FLJ25530

no change

225.00%

 

AK056024

EST

49%

10.60%

 

DR005162

EST

21%

1163.00%

 

AL162511

genomic DNA

49%

26.00%

 

AC096861

genomic DNA

19%

16.00%

 

AC145098

genomic DNA

41%

12.00%

 

AL590482

genomic DNA

86%

0.150%

 

AL121908

genomic DNA

18%

0.0050%

 

AL513318

genomic DNA

47%

0.0002%

 

AC099787

genomic DNA

100%

0.0012%

 

AL354884

genomic DNA

100%

0.0012%

 

AC119800

genomic DNA

50%

0.0003%

 

AC092631

genomic DNA

40%

0.0002%

 

AL138753

genomic DNA

74%

0.0002%

 

AL356608

genomic DNA

76%

0.0020%

 

AC106856

genomic DNA

17%

0.0001%

 

AC012071

genomic DNA

no change

0.0290%

 

AC112717

genomic DNA

39%

0.0019%

 

AP002803

genomic DNA

64%

0.0140%

 

AL158212

genomic DNA

no change

0.0002%

  1. * Abundance relative to β-actin was calculated using a relative quantitation formula : 2-ΔCt x%, where ΔCt is the qRT-PCR cycle number of target gene minus the qRT-PCR cycle number of β-actin at threshold in the same round of qRT-PCR experiment using cDNA from control brains.