Table 1 Primers used for PCR and RT-PCR.
From: Comparative analysis of dinoflagellate chloroplast genomes reveals rRNA and tRNA genes
Primer | Sequence | Amino acid sequence or adjacent rRNA structure |
|---|---|---|
UF | 5'-TTTGGAATCTCAGCTCGATTC | Â |
UR | 5'-CTCGCACCCAAATTGACT | Â |
CD26f1 | 5'-gtGATgagGTCTGtAGTGG | Â |
NICpsbB | 5'-GCNTTYTGGCAYTGGGC | A F W H W A |
petD f3 | 5'-GARCCNGCNTGGCCNAA | E P A W P N |
atpB r1 | 5'-TCNCGGAARTAYTCNGCC | (M) A E Y F R D (in reverse) |
LSU1F | 5'-AGTGATACTGTTCTCTTGAG | 3' helix 46 LSU rRNA |
LSU2R | 5'-GGTTTCATAACTCGGTTGTC | 5' helix 62 LSU rRNA |
LSU3F | 5'-GACAACCGAGTTATGAAACC | 5' helix 62 LSU rRNA |
LSU4R | 5'-ACGTCCAGTGTCATTTCACC | 5' helix 72 LSU rRNA |
SSU1F | 5'-GAAACTTAAAGGTGCTGGTGG | 3' helix 27 SSU rRNA |
SSU2R | 5'-GTTACTAACGATTCCAGCTTC | 5' helix 43 SSU rRNA |