Figure 5

Dot-blot analysis of the duplication of tarIJL in S. aureus N315. The two tarIJL regions in Mu50 were aligned and dot-plot analysis was performed. The homology between these two regions is clearly shown. The high homology also indicates this duplication should not be a remote event. And part of tarL region is less conserved, which indicate that two tarL copies could have different functions. This phenomenon can be used to explain why there is no homologs of tarK in these S. aureus strains. Other S. aureus strains give similar results and are not shown here.