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Table 3 Information on the most abundant contigs. The number of sequences that compose the contigs and the source library, their length, GC content and annotation are indicated. The contig with the highest number of sequences was identified as part of the B. tabaci primary symbiont, Candidatus Portiera aleyrodidarum, based on a partial genomic sequence (gi|32423678|gb|AY268081.1|). This DNA sequence was included in the assembly process. Among the most the abundant contigs were B. tabaci mitochondrial genome sequences (most of them were removed during the preassembly stage)

From: Whitefly (Bemisia tabaci) genome project: analysis of sequenced clones from egg, instar, and adult (viruliferous and non-viruliferous) cDNA libraries

Contig name

Number of sequences

Length (bp)

GC content

EGG

INST

HBT

TYLCV

TOMOV

Annotation

Candidatus

425

31,123*

0.3

22

393

3

2

4

Candidatus

Bt-HInst-045-1-B11-T3_B11

167

598

0.16

8

159

0

0

0

Mitochondria

Bt_TYLCV004_B07

155

2,883

0.48

0

0

51

57

47

Vitellogenin precursor

Bt-HInst-008-1-D2-T3_D02

103

589

0.15

0

103

0

0

0

Mitochondria

Bt-ToMoV-020-1-D2-T3_D02

60

1,660

0.64

0

0

22

13

25

Vitellogenin precursor

Bt-H-024-1-D9-T3_D09

60

1,775

0.47

0

0

43

15

2

Large subunit rRNA

Bt-HInst-032-1-E6-T3_E06

58

792

0.46

2

56

0

0

0

Unknown

Bt-TYLCV-043-1-D2-T3_D02

52

1,922

0.46

0

0

8

20

24

Vitellogenin precursor

Bt-HInst-013-1-H10-T3_H10

47

260

0.38

1

46

0

0

0

Novel

Bt-HInst-003-1-G5-T3_G05

46

970

0.4

0

19

13

9

5

Myosin

Bt-ToMoV-034-1-B10-T3_B10

39

1,039

0.65

0

0

0

30

9

Putative senescence

Bt-ToMoV-023-1-C12-T3_C12

28

542

0.46

0

0

7

12

9

Novel (signal peptide & transmembranal domain)

Bt-TYLCV-030-1-C9-T3_C09

28

1,959

0.44

0

0

3

23

2

Vitellogenin precursor

  1. * In the assembly process the sequence of the whitefly primary endosymbiotic bacteria Candidatus Portiera aleyrodidarum [21] (AY268081.1) was included (see Results, EST assembly into contigs)