Figure 3

RT-PCR analysis of genes related to bone remodeling, muscle development and lipid binding and metabolism. (A) – bone remodeling include BMP4, Biglycan, BSP, (B) – muscle development include Actin, Desmin and (C) – lipid binding and metabolism include AP-2, LPL, Adipsin. (A-C) – Y axis represents the gene expression level (normalized to G3PDH) of the YU (light blue bars), YT (blue bars), OU (pink bars), and OT (purple bars). T-test comparing expression level in treated and untreated age-matched samples was performed – * p value < 0.005, expression levels of all genes analyzed except Biglycan are significant with p value < 0.05. (D) – Y axis represents the ratio between treated and untreated cells from young (blue bars) and old (purple bars) rats.