Figure 3

Snapshots of ConiferEST Web Portals. Panel A: All cDNA libraries of Pinus taeda have been differentially categorized for easy data navigation and comparison. For fast retrieval of individual sequences, users first select the ConiferEST option within the pull-down menu shown in the top portion. Users then enter either the specific sequence name (e.g., FLD1_34_H08.g1_A029), GenBank accession (e.g., CO162374), or GenBank gi number (e.g., 48932915), and click the Go button. Panel B: After choosing one or more libraries from the expandable tree shown in Panel A, the database query interface provides users three options, one of which is "Sequences with putative features". As shown, there is a variety of data filters that can be applied to retrieve putative feature data in terms of users' needs. Panel C: The second option is "Sequences with verified features". Users can not only specify sequences with or without certain verified termini, but also require specific length for verified polyA and/or polyT tails. Panel D: The third option is "Sequence with InterproScan annotation". Users can choose among different InterPro Member databases. They can also conduct advanced field search by text pattern matching. Panel E: Clicking the Submit button shown in the Panel D returns the sortable, tabulated InterProScan results from the database.