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Table 4 Effect of vectors on vector trimming performance by three programs

From: An optimized procedure greatly improves EST vector contamination removal

Vector

#EST tested

Trimming Programs

  

SeqClean

Cross_match

LUCY2

LUCY2(qul)

  

CVS

RVS

CVS

RVS

CVS

RVS

CVS

RVS

PCMV. SPORT-6.1

3000

0.03%

0.03%

3.17%

3.17%

0.30%

0.27%

0.17%

0.17%

PT7T3PAC

3000

0.00%

0.00%

0.23%

0.77%

0.10%

0.07%

0.03%

0.03%

PDNR-LIB

3000

0.30%

0.30%

1.17%

1.17%

0.00%

0.00%

0.03%

0.03%

pGEM-Teasy

2882

38.38%

0.03%

6.45%

7.15%

0.52%

0.52%

0.31%

0.31%

pTriplEx2

2318

0.04%

0.04%

9.66%

9.66%

4.66%

4.87%

5.31%

5.52%

pCR21TOPO

5

0.00%

0.00%

0.00%

0.00%

0.00%

0.00%

0.00%

0.00%

pZL1

700

1.43%

0.00%

3.86%

3.86%

2.71%

2.71%

2.29%

2.29%

pT7Blue

130

0.77%

0.00%

9.23%

9.23%

0.77%

25.38%

0.77%

25.38%

Total

15035

7.50%

0.08%

3.90%

4.14%

1.03%

1.26%

1.04%

1.28%

  1. Contamination rates of ESTs resulted from vector trimming under indicated conditions were grouped by the cloning vectors. The ratio was expressed in percentage showing the proportion of the ESTs using a specific cloning vector remained incompletely cleaned.