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Figure 2 | BMC Genomics

Figure 2

From: A BAC pooling strategy combined with PCR-based screenings in a large, highly repetitive genome enables integration of the maize genetic and physical maps

Figure 2

Representative gel images of BAC pool screening. Primers for umc1658 were used to amplify pool DNA. On average 6 positives per dimension are expected. Amplified PCR products were electrophoresed on 4.5% Super Fine Resolution Agarose gels (A). Each gel contained two dimensions of BAC pools, first gel with plate (PP) and face (FP) pools, second gel with side (SP) and row (RP) pools, and the last gel on the right with column (CP) and diagonal (DP) pools. First and last lane of each tier contains 100 bp ladder. Electrophoresed gel images were scored in tab delimited text format (B). Amplified products were deconvoluted by Resolve script using 3 equations (C). BAC addresses were automatically converted using Lab Convert software (D).

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BMC Genomics

ISSN: 1471-2164

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