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Table 2 Transcription factors used for PCR primer development and BAC pools screening

From: A BAC pooling strategy combined with PCR-based screenings in a large, highly repetitive genome enables integration of the maize genetic and physical maps

Transcription Factor

Map bin location

Functions

Myb or Myc

PCR marker

Copy #

Contig # (# of BACs hit)

GenBank Accession

C1

9.01

Anthocyanin regulation; Transcription factor

Myb

p-C1

2

370 (2)

M37153

PL1

6.04*

Anthocyanin regulation; Transcription factor

Myb

p-phi031, p-umc1014

2

280 (3), 386 (1) 280 (3)

L19495

P1

1.03*

Anthocyanin regulation; Transcription factor

Myb

p-phi095

-

11 (6), 210 (1)

Z11879

MRP1 (= ZmCMT) Myb related protein

8.05

MRP1 gene; one repeat myb transcriptional factor; first transfer cell specific TF

Myb

p-MRP1

1

32 (4), 354 (1)

AJ318518

R2R3

-*

Regulation of secondary metabolism, control of cellular morphogenesis and regulation of meristem formation

Myb

p-R2R3

High

265 (2)

AF474115

O2 (opaque 2)

7.01*

DNA-binding protein; leucine zipper; opaque 2 gene; transcriptional activator. Regulates zein deposition in maize endosperm

Myb

p-Phi112, p-umc1066

-

296 (3) 296 (2)

X16618

Myb2

3.05

Similar to Arabidopsis cdc5 myb protein

Myb

p-Myb2

2

131 (5)

T18844

R1

10.06

Anthocyanin regulation; Transcription factor

Myc

p-R1

-

415 (7)

U93178

SN1-bol3

10.06

Anthocyanin regulation; SN gene; Transcription factor

Myc

p-Sn1

-

415 (6), 421 (1)

X60706

B

2.03*

Anthocyanin regulation; B transcriptional activator; Transcriptional activator

Myc

p-umc1776

-

74 (6)

X70790

Myc7E

-

Regulate iron metabolism in both low- and high-affinity iron transport; Transcription factor

Myc

p-Myc7

-

26 (3)

AF061107

ZmHOX1 (Homeobox1)

8.05

Protein product binds to feedback control element of sh1 promoter

N.A.a

p-umc1309, p-umc1343

1

325 (1), 353 (3) 96 (1), 110 (1), 145 (1), 353 (5)

-

MBF1

4.05, 9.03

Transcriptional factor

N.A.a

p-MBF1

2

162 (1)

T14752

goliath1

4.08

Transcription factor, similar to Drosophila Goliath protein

N.A.a

p-gol1

1

184 (1)

T18797

Golden plant2 (g2)

3.00*

putative transcription factor golden2 gene

N.A.a

p-umc2109

-

111 (7)

-

  1. aN.A.: not available. *Anchor marker.