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Figure 5 | BMC Genomics

Figure 5

From: Gene response profiles for Daphnia pulex exposed to the environmental stressor cadmium reveals novel crustacean metallothioneins

Figure 5

Confirmation of array results. Quantitative-real time (Q-RT)-PCR was used to confirm a subset of genes identified with the microarrays to be regulated in response to cadmium; A) cuticle protein-2, Contig 257, B) 2-domain haemoglobin protein subunit, Contig 262, C) metallothionein, Contig 221) and one gene for which expression was not altered D) serine-threonine kinase, Contig 274 (Table 2). Sequences for primer pairs and probes are provided in Table 3. Expression levels were validated using aliquots sub-sampled from pools of RNA that were used for microarray analysis (technical validation, open triangles) and RNA collected from repeated, independent exposures (biological validation, open bars, mean ± SD, n = 5).

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