Table 4 Amino acid alignment of three D. pulex metallothionein genes against those from other crustaceans (decapods) and from selected insectsa.
Protein domains | |-------------- Beta --------------| |------------------- Alpha --------------------| |
|---|---|
Mtn1 Anopheles gambiae | MPC KC CGN-DC KC TSG---C GSGQPC AT---DC KC AC ASGGC KEKS------------------------------GGCC GK-- |
Mtn2 Anopheles gambiae | MPC KTC VA-DC KC TSP--NC GAGC GC ES---RC TC PC KDGAK----------------------------------EGCC K--- |
MtnA Drosophila melanogaster | MPC P-C GS-GC KC ASQ--ATKGSC NC GS---DC KC G---------------------------------GDKKSA-C GC SE--- |
MtnB Drosophila melanogaster | MVC KGC GT-NC QC SAQ--KC GDNC AC NK---DC QC VC KNGPK----------------------------------DQCC SNK- |
MtnC Drosophila melanogaster | MVC KGC GT-NC KC QDT--KC GDNC AC NQ---DC KC VC KNGPK----------------------------------DQCC KSK- |
MtnD Drosophila melanogaster | MGC KAC GT-NC QC SAT--KC GDNC AC SQ---QC QC SC KNGPK----------------------------------DKCC STKN |
CuMtn2 Callinectes sapidus CRAB | MPC G-C GT-SC KC GSGKCCC GSTC NC TTCPSKQSC SCNDGAC GSAC--------------QC KTSC CC GADC K---C SPC PMK- |
Mtn1 Callinectes sapidus CRAB | MPGPC C ND-KC VC QEG--GC KAGC QC TS----C RC S-PC QKC TSGC--------------KC ATKEEC SKTC TKP-C SCC PK-- |
Mtn2 Callinectes sapidus CRAB | MPDPC C ND-KC EC KEG--EC KTGC KC KS----C RC P-PC DKC SSEC--------------KC TSKEEC SKTC SKP-C SCC P--- |
Mtn1 Scylla serrata CRAB | -PGPC C ND-KC VC KEG--GC KEGC QC TS----C RC S-PC EKC SSGC--------------KC ANKEEC SKTC SKA-C SCC PT-- |
Mtn2 Scylla serrata CRAB | MPDPC C ID-KC DC KEG--EC KTGC KC TS----C RC P-PC EQC SSGC--------------KC ANKEDC RKTC SKP-C SCC P--- |
Mtn1 Potamon potamios CRAB | -PDPC C AEGTC EC EEG--KC KAGC KC TS----C RC S-PC EKC TSEC--------------EC KSKEEC AKNC TKP-C SCC P--- |
Mtn Carcinus maenas CRAB | MPDPC C ID-KC EC KEG--GC KAGC KC TS----C RC T-PC EKC SSGC--------------KC TTKEDC CKTC TKP-C SCC P--- |
Mtn Eriocheir sinensis CRAB | MPDPC C ND-KC EC KEG--KC EAGC KC TS----C RC P-PC EKC SSGC--------------KC GSKEDC CKTC SKP-C SCC P--- |
Mtn Portunus pelagicus CRAB | MPDPC C ID-KC EC KEG--KC EAGC KC TS----C RC P-PC EKC SSGC--------------KC GSKEDC CKTC SKP-C SCC P--- |
Mtn Panulirus argus LOBSTER | MPGPC C ID-KC EC AEG--KC KSGC QC KS----C TC STPC DKC TTAC--------------CC STKEEC ASKC TKP-C KCC P--- |
Mtn Homarus americanus LOBSTER | MPGPC C KD-KC EC AEG--GC KTGC KC TS----C RC A-PC EKC TSGC--------------KC PSKDEC AKTC SKP-C KCC P--- |
Mtn Astacus astacus CRAYFISH | MPGPC C ND-VC EC AAG--GC KTGC VC TS----C RC S-PC DKC TSGC--------------KC PSKEEC AKTC SKP-C ECC P--- |
Mtn1 Daphnia pulex cDNA | MTKD--------------CC QGKC SC GD---NC KC GPNC AQC PAAA----TC AC ATGGEC KC SGNC QC STSC PCK-SACC K--- |
Mtn2 Daphnia pulex cDNA | MPKEC V------------RC QNGC TC GD---DC KC AANC IKC PTASSQGETC KC STPGGCTC GTNC QC GASC VC KASSCC K--- |
Mtn3 Daphnia pulex cDNA | MPNAC C QN-KC SC GSGCNCC QSKC TC GS---GC KC GPNGAPC QNSA-----C IC ATGGG-GC GSDC RC PTSC GC K-TSCC K--- |
Mtn3 Daphnia magna cDNA¥ | MPNVC C QN-NC SC GNGC TCC QSKC TC GS---GC KC GPNGAPC QNSA-----C IC ATGGG-GC GSDC RC PVSC GC K-TSCC K--- |
Cysteine residues | 0 00 0 0 0 0 0 0 1 1 1 1 1 1 1 1 1 1 2 2 2 22 |
1 23 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 34 | |
Metal binding | ** * * * * * * * * * * * * * * ** |