Figure 3

Characteristic features of P. tetraurelia actins, ARPs and ALP. Characteristic features include consensus and drug binding motifs. These are identical within subfamilies wherefore only one isoform of each is shown as an example. The actin signatures (green) are based on the three actin consensus patterns according to PROSITE: ACTINS_1, [FY]-[LIV]-G-[DE]-E-A-Q-x-[RKQ](2)-G; ACTINS_2, W-[IV]-[STA]-[RK]-x-[DE]-Y-[DNE]-[DE] and ACTINS_3, [LM]-[LIVM]-T-E-[GAPQ]-x-[LIVMFYWHQ]-N-[STAQ]-x(29-N-[KR]. Not all isoforms contain the typical consensus pattern found in most of the actin genes described in other species. Also the three residues mediating the binding of phalloidin (G158, R177 and D179) are not present in every subfamily (red). The same is true for the residues (blue) relevant for the binding of latrunculin A (D157, R183, D184, R210, D211, K213, E214 and K215).