Figure 5

Effect of inhibition of the MAP kinase pathways on the regulation of gene expression by TGF-β in A549 cells. The cells were grown to 90% confluence, washed with serum free medium, treated with 10 μM p38 inhibitor (SB203580), ERK inhibitor (PD98059) or JNK inhibitor 1 hour prior to treatment with TGF-β. The cells were harvested after 18 hours following TGF-β treatment and processed for qRT-PCR as described in Figure 3.