Figure 2

Microsatellite associated sequence amplification (MASA) performed using oligos based on varying lengths of GACA/GATA repeats and cDNA from different sources (A-D). The amplified transcripts ranged from 0.15 kb to 1.8 kb. MASA using GACA repeat with cDNA from different somatic and gonadal tissues is given in (A) and cDNA from spermatozoa from 4 animals in (B). Similarly, MASA using GATA repeats and cDNA from different somatic tissues (C) and spermatozoa is shown in (D). Note the tissue and spermatozoa-specific transcript profiles generated by GACA and GATA repeats. GATA did not detect any transcripts in lung and heart.