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Table 2 Experimental candidates with corresponding E-transcript proportions based on EST and experimental data. EST ratios are given as absolute EST counts. Column 'predicted' lists the E-transcript proportions obtained from the sequence-based prediction. Grey shaded values mark the cases where NAGNAG alternative splicing was validated (avg [minor isoform in organs, seedlings] > 2× avg [error]). At4g35785 is lacking EST data. Therefore, an EST-based E-transcript frequency cannot be shown and is indicated by '-'.

From: Alternative splicing at NAGNAG acceptors in Arabidopsis thaliana SR and SR-related protein-coding genes

 

E-transcript (%)

Gene

EST

Predicted

Experiments

RS41

23

7

16.7 ± 0.6

SR33/SCL33

0

48

29.9 ± 0.7

SR45i7

0

48

0.6 ± 0.5

SR45i9

0

7

0.4 ± 0.6

SRZ22a

100

98

99.5 ± 0.6

CID8

100

97

99.1 ± 0.5

At1g76940

100

81

98.8 ± 0.9

At2g24350

0

7

3.2 ± 1.5

ATRZ-1A

100

83

98.9 ± 1.4

At3g54230

0

25

2.3 ± 0.5

At4g35785

-*

98

97.4 ± 0.3

At5g09880

100

98

99.0 ± 0.7

At5g59950

0

7

2.8 ± 0.4

U11–35K

0

17

8.5 ± 0.9

U2AF65

0

17

5.8 ± 0.6