Novel and nodulation-regulated microRNAs in soybean roots

BMC Genomics

Table 4 Relative abundance of selected miRNA reads in the control and Bj libraries^a

	Library
	Control		Bj
miRNA Family	Number of reads	Frequency^b	Number of reads	Frquency^b	Relative abundance (Bj/Control)
171^d	12	0.18	23	0.27	1.5
482	21	0.31	39	0.46	1.5
1507	22	0.33	38	0.44	1.4
390	12	0.18	19	0.22	1.3
160	39	0.58	60	0.70	1.2
1509	216	3.21	318	3.72	1.2
1508	28	0.42	40	0.47	1.1
396	54	0.80	77	0.90	1.1
159	584	8.68	831	9.73	1.1^c
164	25	0.37	35	0.41	1.1
166	50	0.74	68	0.80	1.1
168	29	0.43	39	0.46	1.1
393	30	0.45	39	0.46	1.0
894?	7	0.10	9	0.11	1.0
1510	23	0.34	29	0.34	1.0
156	37	0.55	46	0.54	1.0
171	60	0.89	72	0.84	1.0
1511	11	0.16	13	0.15	0.9
167	52	0.77	61	0.71	0.9
1512	8	0.12	9	0.11	0.9
172	184	2.74	205	2.40	0.9
482?	20	0.30	22	0.26	0.9
169	31	0.46	31	0.36	0.8
162	6	0.09	6	0.07	0.8
319	50	0.74	45	0.53	0.7
1513	25	0.37	20	0.23	0.6

^a Only reads with a total abundance of ≥ 10 in both libraries combined are presented.
^b Frequency is calculated as the number of reads representing the family per thousand reads in the corresponding library that matched known soybean sequences. Number of reads in Control and Bj libraries that matched known soybean sequences were 67248 and 85406 respectively.
^cThe difference in read counts is statistically significant (P < 0.05) based on Poisson's distribution analysis.
^d The precursors of these miRNAs seems to be evolutionarily related, but the mature miRNAs are different from each other and we classified them as a novel miRNA.

ISSN: 1471-2164