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Table 1 sRNAs transcripts observed in P. aeruginosa PAO1

From: Genome-wide search reveals a novel GacA-regulated small RNA in Pseudomonas species

Intergenic region a

Coordinates in PAO1 genome (bp)

5' gene

3' gene

sRNA

  

Length (bp)

Dir.b

Length (bp)

Dir.b

Predicted length (nt)c

Observed length (nt)d

Dir.g

491

912780–913085

1185

<

486

>

90

100, 200

>

622

1140860–1141267

1146

>

465

>

 

200, 300

?

645

1204782–1205770

1149

>

1767

>

300 ± 10

300

<

888 = ffs

1668833–1669085

378

>

867

<

113

90

>

1059

1996807–1997508

1659

<

1041

<

 

150, 200, 300

?

1466

2918212–2918965

1041

>

534

<

 

300

?

1559

3112151–3112876

519

>

900

>

 

250, 300e

?

1698 = rgsA

3318657–3318881

1134

>

777

<

120 ± 10

100, 300

>

1714

3360654–3360873

1386

<

3474

>

 

200

?

1887

3705161–3705888

1995

<

603

<

210 ± 10

200

<

2315

4536493–4536919

660

<

1122

<

160 ± 10

180

<

2510 = rnpB

4956029–4956732

452

<

849

<

208

200

<

2626

5196833–5197184

1464

>

576

>

 

200

>

2667 = prrF1,2

5283906–5284368

798

>

888

<

110, 110

100, 110 f

>>

  1. a Transcripts that were also found by Livny et al. [20] are shown in boldface
  2. b Dir., direction of flanking genes
  3. c Lengths of transcripts described in other studies are indicated by their experimental values; otherwise, predicted lengths are estimates ± 10 nt
  4. d Bands that were observed in cells grown in NYB, GGP or MME medium; estimated size ± 10 nt
  5. e Not tested in GGP and MME medium
  6. f No transcript was observed in cells grown in NYB or GGP in exponential phase; transcripts were present in stationary phase cells grown in all media
  7. g Dir., direction of sRNA genes. When rho-independent terminators are predicted to be present, the direction of the sRNA is annotated (> or <). When the direction is unknown, this is indicated by a question mark