Table 4 Primers used for probes, sequencing and gene expression.
Primer | Sequence (5'-3') | Position | Comments |
|---|---|---|---|
oOV4F | GCGCCATACTGGACAAGACAG | Exon 2 | cDNA amplification Sasa-DAB |
oOV6R | CTGCTGCAGATTCAGCAACAT | 3' UTR | cDNA amplification Sasa-DAB |
DAB40F | ATGTCGATGTCTATCTTCTG | Exon 1 | cDNA amplification Sasa-DAB |
DAB489R | GTACCAGTCCCCGTTAGCCAG | Exon 3 | cDNA amplification Sasa-DAB |
DAA66F | TGCTGGCAGGTGTATGCAGAA | Exon 1 | cDNA amplification Sasa-DAA |
DAA660R | GAATGTTCCGGCAGCCACTCC | Exon 4 | cDNA amplification Sasa-DAA |
DBA19F | TCACTGGAGCCGTTTGCACTT | Exon 1 | cDNA/gDNA amplification Sasa-DBA |
DBA51F | CATCATGAAATTCACTTTATC | Exon 2 | cDNA/gDNA amplification Sasa-DBA |
DBA304R | CTTTGATTTTTGGTATTTTCG | Exon 2 | cDNA/gDNA amplification Sasa-DBA |
DBA660R | CCCCAGCAGCCCAAGAGTGAG | Exon 4 | cDNA/gDNA amplification Sasa-DBA |
DBB98F | CTCTGAGACTTGATTATGTAT | Exon 1 | cDNA/gDNA amplification Sasa-DBB |
DBB177F | TTGATTCCTCAGATGAAGATT | Exon 2 | cDNA/gDNA amplification Sasa-DBB |
DBB441R | CCATAAACATCTCTGTTGCGT | Exon 2 | cDNA/gDNA amplification Sasa-DBB |
DBB825R | CAGTAGATTTCGCCCAGTAGA | Exon 4 | cDNA/gDNA amplification Sasa-DBB |
DA847F | GATGGCAAAGAGGAAAGTGAG | 3' UTR | FAM labeled minisatellite primer for Sasa-DAA |
DA1054R | TTGTTATGCTCTACCTCTGAA | 3' UTR | Minisatellite primer for Sasa-DAA |
Sp6 | ATTTAGGTGACACTATA | pTARBAC2.1 | End sequencing BAC vector Sp6 end |
T7 | TAATACGACTCACTATAGGG | pTARBAC2.1 | End sequencing BAC vector T7 end |
M13F | GTTTTCCCAGTCACGAC | pUC19 | DNA plasmid sequencing primer |
M13R | CAGGAAACAGCTATGAC | pUC19 | DNA plasmid sequencing primer |
EF1A_F | CACCACCGGCCATCTGATCTACAA | Acc. No. AF321836 | Real-time PCR Elongation factor 1-alpha |
EF1A_R | TCAGCAGCCTCCTTCTCGAACTTC | Acc. No. AF321836 | Real-time PCR Elongation factor 1-alpha |
DAB566F | ATGGTGGAGCACATCAGCC | Exon 3 | Real-time PCR Sasa-DAB |
DAB620R | CTCAGCCTCAGGCAGGGAC | Exon 4 | Real-time PCR Sasa-DAB |
DBB418F | GCCATATGCTAATCGGATCTAC | Exon 3 | Real-time PCR Sasa-DBB |
DBB500R | ACACACAAGGCTGGAGTCACT | Exon 4 | Real-time PCR Sasa-DBB |
DBA538F | GCATCGCATGGGGCAAACAG | Exon 3 | Real-time PCR Sasa-DBA |
DBA641R | ATTCACAAAGCAGATGAGGGTG | Exon 4 | Real-time PCR Sasa-DBA |
DCA277F | GATTGTGTCGGGATGCCTTG | Exon 2 | Real-time PCR Sasa-DCA |
DCA357R | CTCCTCAGCCCTGGGGTAGAT | Exon 3 | Real-time PCR Sasa-DCA |
DDA225F | AGTTGCAGAGAGCCGAAGAGC | Exon 2 | Real-time PCR Sasa-DDA |
DDA317R | TTCACTTCATCCTTGGCATACA | Exon 3 | Real-time PCR Sasa-DDA |