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Figure 2 | BMC Genomics

Figure 2

From: ERK1 and ERK2 MAPK are key regulators of distinct gene sets in zebrafish embryogenesis

Figure 2

Comparison of the ERK1 and ERK2 knockdown gene expression profiles in time. (A,B) Venn diagrams showing the gene expression profiles in time for ERK1 and ERK2 knockdown respectively. (C,D): graphs representing the number of genes that showed changes in expression, as well as their fold of change (greater than 1, 2, 4, 8, and 16 fold changes) upon knockdown of ERK1 (C; 4.5 and 8hpf) or ERK2 (D; 3.5, 4.5, 6 and 8hpf) at (p < 10-5). Knockdown of ERK2 affects the expression of more genes, and with a higher fold of changes than knockdown of ERK1, but increased in time for both conditions. (E,F); Venn diagrams, comparing ERK1 versus ERK2 expression profiles at 30% epiboly and 80% epiboly respectively. The signatures of ERK1 and ERK2 morphants are split in up- and down-regulated genes in the graphs (C,D) and Venn diagrams (E,F). By doing so, the Venn diagrams also shows the numbers of specifically up and down regulated genes, common up and down regulated genes, and two anti-correlated gene pools (up-regulated in ERK1MO down-regulated in ERK2MO and down-regulated in ERK1MO up-regulated in ERK2MO); yellow = up-regulated by ERK1MO (ratio > 1), blue = down-regulated by ERK1MO (ratio <1), red = up-regulated by ERK2MO (ratio > 1), green = down-regulated by ERK2MO (ratio <1). (G) A model for the downstream ERK1 and ERK2 signaling pathway, showing distinct functions for ERK1 and ERK2 in gene regulation. Panels (A) represents ERK1 specific genes, (B) ERK2 specific genes, (C) common genes, and D1 and D2, are representing two different anti-correlated gene pools as sub-populations of the common gene pool C.

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