Skip to main content

Table 5 Parameters of individual and composite linkage maps of white spruce and black spruce.

From: Enhancing genetic mapping of complex genomes through the design of highly-multiplexed SNP arrays: application to the large and unsequenced genomes of white spruce and black spruce

Mapping parameters

Cross/Parents for white spruce (Picea glauca)

Cross/Parents for black spruce (species complex Picea mariana × P. rubens)

 

Parents

Composite

Parents

Composite

 

♀80112

♂80109

 

♀11307-03 [♀83 × ♂425]

♂425

 

Total number of available markers

525

597

1039

587

563

1260

Number of distorted markersb

7

11

17

12

3

28

Total number of markers without segregation distortion

518

586

1022

575

560

1232

Total number of assigned markers

509

581

957

534

542

1064

   Number of AFLP loci

256

299

581

247

242

679

   Number of RAPD loci

0

0

0

2

1

3

   Number of SSR loci

9

10

13

27

27

45

   Number of ESTP gene loci

22

23

35

26

30

34

   Number of SNP gene loci

222

249

328

232

242

303

Number of positioned markers (%)

483 (91.0)

523 (90.0)

821 (85.8)

461 (86.3)

479 (88.4)

835 (78.5)

   Number of AFLP loci

242

257

461

185

188

469

   Number of RAPD loci

0

0

0

2

1

2

   Number of SSR loci

8

9

12

25

27

36

   Number of ESTP gene loci

19

19

31

23

27

30

   Number of SNP gene loci

214

238

317

226

236

298

Number of positioned accessory markers

4

3

9

0

6

22

Number of major linkage groups (nb of sub-groups) (n > 10 markers)

12 (4c)

12 (1c)

12

12 (2c)

12 (2c)

12

Number of minor linkage groups (3 ≤ n ≤ 10 markers)

0

1

0

0

0

0

Number of unlinked markers

9

5

65

41

18

168

Total observed map length GF, cM (Kosambi)

2146.1

2283.6

2304.2

1833.5

1814.1

1849.8

Average map density, cM (Kosambi)

4.4

4.4

2.8

4.0

3.8

2.2

Average size for major linkage groups, cM (Kosambi)

134.1

163.1

192.1

130.9

129.5

154.1

Expected map length Ge, cM (Kosambi)

3204.5

3569.9

-d

4009.4

3424.7

-d

Map coverage Ce (%)

98.3

98.4

-d

97.7

98.1

-d

  1. aFor individual linkage maps, only markers segregating 1:1 or 1:1:1:1 were used.
  2. bBonferroni correction: P ≤ 0.01/number of loci.
  3. cNumber of linkage group composed of 2 sub-groups having more than 10 markers.
  4. dCould not be calculated due to the merging of data.