Figure 5

C-terminal sequence motif identified by MEME analysis. A. Sequence logo representing actual matches to the motif position-specific scoring matrix in An. gambiae and Ae. aegypti. The motif is present in 29 An. gambiae RR-2 genes on chromosome 2L and in 65 annotated genes in Ae. aegypti. B. Sequence logo representing a modified version of the motif that is reduced by one position and permits indels. Arrows indicate sites under negative selection at P < 0.05, as determined by single-likelihood-ancestor counting [32]. Overall Ka/Ks for the modified motif was estimated to be 0.42 [95% CI: 0.39, 0.65].