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Figure 3 | BMC Genomics

Figure 3

From: Developing and applying a gene functional association network for anti-angiogenic kinase inhibitor activity assessment in an angiogenesis co-culture model

Figure 3

NHDF passage dependent angiogenic supportiveness in cord formation assay. P6 NHDFs from a neonatal donor, P5 and P12 NHDFs from an adult donor were tested for their angiogenesis supportiveness in the cord formation assay (A). Cultures were performed either with or without 20 ng/ml exogenously added VEGF. Cords were visualized by human CD31 staining (green). Both early passage neonatal and adult NHDF cells are more supportive for cord formation than the late passage adult NHDFs, with the late passage NHDF cells being non-permissive. (B). Clustering analysis of genes involved in angiogenesis. P6 NHDFs from a neonatal donor (Neo P6), P4 NHDFs from an adult donor (Ad P4), and P13 NHDFs (Ad P13) from the same adult donor were assayed for basal gene expression using the Affymetrix GeneChip platform. 787 nonredundant genes were identified as common differentially expressed genes between the two early passage NHDF and the late passage NHDF cells. Enrichment testing was used to identify significant Biological Process (BP) categories. Representative angiogenesis BP categories are shown in a heatmap visualization.

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BMC Genomics

ISSN: 1471-2164

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