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Figure 5 | BMC Genomics

Figure 5

From: Combination of native and denaturing PAGE for the detection of protein binding regions in long fragments of genomic DNA

Figure 5

Confirmation of the protein binding regions with individual DNA fragments. F202, a 202 bp Bgl II-Nde I fragment derived from -1.8 kb region partially overlapping with a 359 bp Alu I fragment; F395, a 395 bp PstI fragment derived from -6.3 kb region partially overlapping with a 737 bp Alu I fragment; F514, a 514 bp Pst I fragment derived from -6.8 kb region overlapping with a 200 bp Bsu RI fragment (for exact nucleotide positions see Fig. 2). NE, PC12 nuclear extract. A ten-fold excess of the same, but unlabeled fragment was used as a competitor. Arrowheads point at bands resulting from sequence-specific protein-DNA interactions. Note the smearing in case of F514, which suggests dissociation of complex(es) during electrophoresis. The asterisk indicates a weak band, corresponding to a putative specific complex.

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