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Figure 6 | BMC Genomics

Figure 6

From: Combination of native and denaturing PAGE for the detection of protein binding regions in long fragments of genomic DNA

Figure 6

The effect of the poly (dI-dC) concentration on the mobility shift of restriction fragments. A poly (dI-dC) concentration range from 100 to 800 μg/ml was tested with 2-fold differences. At each concentration, the incubation of labeled restriction fragment with PC12 nuclear extract (NE) was performed using two different poly (dI-dC) preparations with the average sizes 250 and 500 bp. C1, a 202 bp Bgl II-Nde I fragment and C2, a 384 bp Nde I-Mph I fragment derived from -1.8 kb and -1.6 kb regions of the rat p75NTR promoter, respectively. Arrowheads point at the major specific DNA-protein complexes. M, 32P-labeled 100 bp ladder (Gibco-BRL).

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