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Figure 7 | BMC Genomics

Figure 7

From: Combination of native and denaturing PAGE for the detection of protein binding regions in long fragments of genomic DNA

Figure 7

EMSA experiments with different preparations of DNA fragments. A 275 bp fragment (F275), derived from L1 antisense promoter [20], was gel isolated by using QIAquick® Gel Extraction Kit (panel A) or gel-diffusion method described here (panel B), radiolabeled and incubated with (+) or without (-) nuclear extract. The presence (+) or absence (-) of competitor, a 10-fold excess of the unlabeled fragment, is shown. Note that specific mobility shift, shown by arrowhead, is visible only in panel B.

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