Skip to main content
Figure 1 | BMC Genomics

Figure 1

From: A simple and accurate SNP scoring strategy based on typeIIS restriction endonuclease cleavage and matrix-assisted laser desorption/ionization mass spectrometry

Figure 1

Schematic summary of the RFMP genotyping strategy. PCR was done with primers designed to introduce a typeIIS restriction endonuclease recognition sequence (Fok I = Bst F5I; GGATG) 9 bases ahead of the polymorphism site. The enzymatic cleavage of the products leads to excision of two oligonucleotide fragments (7 mer and 13 mer) containing the variation site, and then masses of the resulting oligonucleotide fragments were examined by MALDI-TOF MS. Cleavage sites of Fok I and BstF 5I, an isoschizomer for Fok I, are indicated by filled and blank triangles, respectively, and restriction endonuclease recognition site and primers by shaded bar and shaded arrows, respectively. One-base gap replaced by the artificial sequences and potential SNP site are denoted by blank space and a bold italic letter, respectively.

Back to article page