Table 2 Expected masses of oligonucleotides resulting from restriction enzyme cleavage of PCR products for scoring 5 SNPs in MTHFR gene by RFMP method
SNPs | Variation | Seuences of restriction fragmentsa | Expected mass (Da) | ||
|---|---|---|---|---|---|
| Â | Â | 7 mer | 13 mer | 7 mer | 13 mer |
rs1801133 | C | CGGGAGc | ATCGgCTCCCGCA | 2226.4 | 3975.6 |
| Â | T | CGGGAGt | ATCGaCTCCCGCA | 2241.4 | 3959.6 |
rs2066462 | C | TCTCAGc | GGGCgCTGAGAGC | 2136.4 | 4120.6 |
| Â | T | TCTCAGt | GGGCaCTGAGAGC | 2151.4 | 4104.6 |
rs1994798 | C | CAAATAc | AGATgTATTTGCA | 2153.4 | 4068.6 |
| Â | T | CAAATAt | AGATaTATTTGCA | 2168.4 | 4052.6 |
rs2066470 | C | GGACCCc | GCTCgGGGTCCAG | 2146.4 | 4071.6 |
| Â | T | GGACCCt | GCTCaGGGTCCAG | 2161.4 | 4055.6 |
rs1801131 | A | GTGAAGa | ACTTtCTTCACTG | 2249.4 | 3955.6 |
| Â | C | GTGAAGc | ACTTgCTTCACTG | 2225.4 | 3980.6 |