Figure 5
From: Genetic marker anchoring by six-dimensional pools for development of a soybean physical map
Anchoring paralogous sequences onto the physical map by 6-D pools and confirmed by FISH. (a) Paralogous sequences of PCR product. The pink arrow shows nucleotide variations in a sequence read. (b) Alignment between homoeologous BACs. Blue lines show some regions with inconsistent alignment between the two BACs (c) FISH mapping of homoeologous BACs (Gm_WBb0130B22 and Gm_WBb0060P19) anchored by marker 13617. The leftmost panel shows the entire karyotype of a chromosome spread prepared from mitotic root tip cells. The probe for BAC Gm_WBb0130B22 (abbreviated as 130B22) was labeled with green fluorophore and Gm_WBb0060P19 (abbreviated as 60P19) was labeled with red fluorophore; overlap therefore appears as yellow. Right panels are enlarged fields in which the left panels show the merged channels, the middle panels show the green (60P19) channel and the right panels show the red (130B22) channel. The 60P19 probe localizes to two pairs of strong spots (corresponding to the duplicated chromatids of homologous chromosomes) as well as to two pairs of less intense signal that overlap with the 130B22 loci. The yellow arrows indicate overlapping spots.