Figure 2
From: Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley
Locus representation in amplified DNA relative to unamplified DNA. Four 1H-specific loci and one locus from each of the remaining chromosomes were examined for representation by real-time quantitative PCR with gene-specific primers. The RT PCR was run on amplified 1H chromosomes (MDA 1H), amplified 2H – 7H chromosomes (MDA 2H-7H), amplified Akcent DNA (MDA Akcent) and unamplified Akcent genomic DNA (Akcent).