Figure 3
From: Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley
Analysis of the product of multiple displacement amplification on flow-sorted chromosomes. (a) 500 ng of the product were analyzed by pulsed field gel electrophoresis in 1% agarose gel, 0.25 × TBE at 12.5°C, 6 V/cm and 5–15 s switch time ramp for 15 hours. Lambda ladder was used as a size standard. (b) 5 μg of the product were analyzed in 1.5% agarose gel in 0.5 × TBE and (c) after transfer on nylon membrane hybridized with barley genomic DNA. (d) Dot blot of barley genomic DNA (Non-MDA) and amplified DNA of all barley chromosomes (MDA) hybridized with barley genomic DNA.