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Figure 3 | BMC Genomics

Figure 3

From: High accuracy mass spectrometry analysis as a tool to verify and improve gene annotation using Mycobacterium tuberculosis as an example

Figure 3

Example of MS-friendly database entry for N-terminal prediction validation. This entry represents the protein Rv2253 (Conserved hypothetical protein) which is 167 amino acids long. Analysis with the tool SignalP v3.0 resulted in the prediction of the sequence A27-A28-A29 (underlined) as a possible cleavage site of signal peptidase I. Therefore, the predicted N-terminal peptide is inserted after a J (box). In addition, we also appended all peptides possible from position -25 until +7 from the predicted signal peptidase cleavage site. In this case, we not only identified the predicted N-terminal peptide starting in E30 (underlined after box) but also a second peptide starting on amino acid A28 (last underline – see Figure 1 for MS/MS data) representing a possible N-terminal alternative option.

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