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Figure 2 | BMC Genomics

Figure 2

From: Global mRNA expression analysis in myosin II deficient strains of Saccharomyces cerevisiae reveals an impairment of cell integrity functions

Figure 2

(A) Genetic disruption of the SLT2/MPK1 gene induces lethality in a myo1Δ strain. Wild type, myo1Δ, slt2Δ and myo1Δ slt2Δ pRS316-MYO1 strains were grown in CSM (1 mg/ml) 5-FOA for three days at 26°C. (B)Suppression of Nikkomycin Z hypersensitivity in myo1Δ strains overexpressing the ribosomal protein genes RPL30 and RPS31. Wt, myo1Δ, myo1Δ pRS316-RPL30 and myo1Δ pRS316-RPS31 strains were grown CSM or CSM URA- in presence or absence of 6.25 μM Nikkomycin Z in 2% glucose or 2% galactose for 48 hours at 26°C. Percent of Nikkomycin Z resistance was calculated as: (OD600 nm treated/OD600nm untreated) × (100). Dark gray histograms represent cells where expression of the plasmid is repressed with 2% glucose and light gray histograms represent cells where expression of the plasmid gene is induced with 2% galactose. (C) Determination of the activation of the cell integrity pathway in myo1Δ strains overexpressing the ribosomal protein genes RPL30 and RPS31. Wild type, myo1Δ, wildtype pRS316-RPL30, wild type pRS316-RPS31, myo1Δ pRS316-RPL30 and myo1Δ pRS316-RPS31 strains were grown in CSM or CSM URA- in the presence of 2%glucose or 2% galactose. Equal amounts of protein (75 μg) were analyzed by Western blot as described in the Methods section. The phosphorylated levels of Slt2p were observed using a mouse monoclonal antibody against phospho-p42/p44 Slt2p (p-Slt2p). The membrane was stripped and reprobed with a monoclonal antibody against Pgk1p as a loading control.

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