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Table 2 Quantitative real-time RT-PCR analysis of selected transcripts.

From: Gene expression patterns of sulfur starvation in Synechocystis sp. PCC 6803

A

Gene

1 hr

3 hr

6 hr

 

rRNA

trpA

lysC

rRNA

trpA

lysC

rRNA

trpA

lysC

cpcA

-1.95 ± 0.23

-2.79 ± 0.01

-2.35 ± 0.01

-1.66 ± 0.11

-2.28 ± 0.17

-2.23 ± 0.06

-1.40 ± 0.21

-1.38 ± 0.02

-1.95 ± 0.04

psbA2

-3.08 ± 0.10

-2.97 ± 0.02

-2.52 ± 0.02

-2.03 ± 0.04

-2.07 ± 0.17

-2.01 ± 0.05

-0.94 ± 0.17

-0.45 ± 0.02

-1.02 ± 0.04

nblA2

0.12 ± 0.10

1.20 ± 0.20

1.14 ± 0.15

3.77 ± 0.03

-0.88 ± 0.09

0.25 ± 0.23

2.98 ± 0.09

n.d.

1.99 ± 0.21

cysW

0.80 ± 0.12

n.d.

n.d.

6.06 ± 0.10

1.41 ± 0.13

2.54 ± 0.25

4.56 ± 0.38

n.d.

3.57 ± 0.43

B

Gene

1 hr

3 hr

6 hr

hoxH

0.65 ± 0.06

0.83 ± 0.07

0.06 ± 0.05

hoxE

0.52 ± 0.03

0.72 ± 0.05

-0.14 ± 0.06

hypA1

0.22 ± 0.01

0.13 ± 0.06

-0.21 ± 0.07

hypB1

-0.01 ± 0.09

-0.31 ± 0.07

-0.86 ± 0.06

lexA

0.52 ± 0.08

-0.4 ± 0.09

-0.98 ± 0.08

  1. A. Transcript abundances of significantly induced nblA2(ssl0453), cysW(slr1454) and repressed cpcA(sll1578), psbA2(slr1311) genes, relative to stable RNA, trpA, or lysC, as endogenous references. B. Measurements of transcript abundances of hydrogenase-related genes using lysC as an endogenous reference. Mid-exponential cells were harvested and fixed in phenol-ethanol stop solution before sulfate withdrawal (time 0) and 1, 3, 6 hr after sulfate withdrawal. Total RNA was isolated and reverse transcribed. The relative abundances of each transcript were measured by SYBR Green real-time PCR and analyzed according to the comparative C t method. The data are presented as transcript abundances, normalized to a reference mRNA, in a time-point sample relative to control (time 0) on the log2 basis. Errors are the pooled s.e.m. (n = 3). "n.d." – not determined.