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Figure 3 | BMC Genomics

Figure 3

From: Deciphering downstream gene targets of PI3K/mTOR/p70S6K pathway in breast cancer

Figure 3

Effects of rapamycin and Ly294002 treatments on phosphorylation of p70S6KThr389 in breast cancer cell lines. The cells were treated with A) 100 nM rapamycin and B) 50 μM Ly294002 for 24 hours and the phosphorylation status of p70S6K Thr389 was detected by Western immunoblotting. The plus (+) sign indicates the inhibitor-treated samples and the minus (-) sign the non-treated samples. Both rapamycin and Ly294002 blocked Thr389 phosphorylation of p70S6K. The total p70S6K expression was used as a reference.

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