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Table 1 Genomic Citrus clementina BAC Libraries

From: Development of genomic resources for Citrus clementina: Characterization of three deep-coverage BAC libraries and analysis of 46,000 BAC end sequences

Library

CCL1

CCER1

CCH3

Vector

pECBAC1

pECBAC1

pECBAC1

Partial digest enzyme

MboI

EcoRI

HindIII

Cloning site

BamHI

EcoRI

HindIII

Average insert size

124 kb

127 kb

132 kb

N° of clones

19,200

18,432

18,432

Missed wellsa

0.57%

0.37%

0.09%

Blue coloniesb

0.78%

1.46%

0.19%

  1. a No bacterial growth detected
  2. b Presence of vector but no insert