Figure 2
From: Amplification biases: possible differences among deviating gene expressions
Quality of the somatic and embryonic amplified products. 125 ng of each amplified material (aRNA or cDNA) has been spotted onto a membrane and hybridised with DNA probes encoding exogenous (CG03) or endogenous controls (somatic: EF1α, L23a, cytochrome oxidase III; embryonic: IFN-tau). Each replicate (1 to 3) has been generated independently with each protocol (IVT or PCR) on each tissue (A: brain, ovary; B: ovoid, tubular and filamentous bovine embryos). PolyA+ RNA was used as standard for somatic tissues only (A).