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Table 1 Libraries, tissue sources and spruce species for sequences described in this study

From: A conifer genomics resource of 200,000 spruce (Picea spp.) ESTs and 6,464 high-quality, sequence-finished full-length cDNAs for Sitka spruce (Picea sitchensis)

cDNA Library

Tissue/Developmental stage

Species (genotype)

WS-ES-A-1a

Young shoots harvested from 25-year old treesd.

P. glauca (PG-29)

WS-PS-A-2a

Flushing buds, young shoots and mature shoots harvested from 25-year old treesd.

P. glauca (PG-29)

WS-X-A-3a

Early (June 15th), mid (July 10th) and late (August 17th) season outer xylem harvested from 25-year old treesd.

P. glauca (PG-29)

IS-B-A-4a

Bark tissue (with phloem and cambium) harvested after razor blade wounding and treatment with 0.01% methyl jasmonate. Tissue was collected 0 (untreated), 3, 6 and 12 h post-treatmente.

P. glauca × P. engelmannii (Fal-1028)

SS-R-A-5a

Young growth (terminal 1–3 cm) and mature growth (distal to terminal 1–3 cm) rootse.

P. sitchensis (Gb2-229)

WS-PP-A-6a

Early (June 15th), mid (July 10th) and late (August 17th) season phloem harvested from 25-year old treesd.

P. glauca (PG-29)

IS-B-A-7a

Bark tissue (with phloem and cambium) harvested after razor blade wounding and treatment with 0.01% methyl jasmonate. Tissue was collected 24 h, 2 d, 4 d and 8 d post-treatmente.

P. glauca × P. engelmannii (Fal-1028)

WS-PS-N-A-8b

Flushing buds, young shoots and mature shoots harvested from 25-year old treesd.

P. glauca (PG-29)

WS-X-N-A-9b

Early (June 15th), mid (July 10th) and late (August 17th) season outer xylem harvested from 25-year old treesd.

P. glauca (PG-29)

IS-B-N-A-10b

Bark tissue (with phloem and cambium attached) harvested after razor blade wounding and treatment with 0.01% methyl jasmonate. Tissue was collected 0 h (untreated), 3 h, 6 h, 12 h, 24 h, 2 d, 4 d and 8 d post-treatmente.

P. glauca × P. engelmannii (Fal-1028)

SS-R-N-A-11b

Young growth (terminal 1–3 cm) and mature growth (distal to terminal 1–3 cm) rootse.

P. sitchensis (Gb2-229)

WS-PP-N-A-12b

Early (June 15th), mid (July 10th) and late (August 17th) season phloem harvested from 25-year old treesd.

P. glauca (PG-29)

SS-IB-A-FL-13c

Bark tissue (with phloem and cambium attached) harvested after continuous feeding by Pissodes strobi weevils. Tissue was collected 2, 6 and 48 h post-treatmente.

P. sitchensis (FB3-425)

SS-IL-A-FL-14c

Green portion of leader tissue harvested after continuous feeding by Choristoneura occidentalis budworms. Tissue was collected 3 h, 6 h, 12 h, 24 h, 52 h, 4 d, 6 d, 8 d and 10 d post-treatmente.

P. sitchensis (FB3-425)

SS-IB-A-FL-15c

Bark tissue (with phloem and cambium attached) harvested after continuous feeding by P. strobi weevils. Tissue was collected 2, 6 and 48 h post-treatmente.

P. sitchensis (FB3-425)

WS-SE-A-16a

Somatic embryo tissue harvested at the callus stage, and after 2, 4 and 6 weeks of growth on media supplemented with abscisic acid and indole-3-butyric acid.

P. glauca (I-1026)

WS-MC-A-17a

Cones harvested from 25-year old treesd

P. glauca (11)

WS-SE-N-A-18b

Somatic embryo tissue harvested at the callus stage, and after 2, 4 and 6 weeks of growth on media supplemented with abscisic acid and indole-3-butyric acid.

P. glauca (I-1026)

WS-SE-N-A-19b

Somatic embryo tissue harvested at the callus stage, and after 2, 4 and 6 weeks of growth on media supplemented with abscisic acid and indole-3-butyric acid.

P. glauca (I-1026)

WS-MC-N-A-20b

Cones harvested from 25-year old treesd

P. glauca (11)

  1. aStandard cDNA library; bNormalized cDNA library; cFull-length cDNA library; dField site located at Kalamalka Research Station in Vernon, British Columbia; eOne- or two-year old trees grown in potted soil under greenhouse conditions at the University of British Columbia