Skip to main content
Figure 4 | BMC Genomics

Figure 4

From: Identification of protein secretion systems and novel secreted proteins in Rhizobium leguminosarum bv. viciae

Figure 4

The tatC mutant of R. l . bv. viciae 3841 is altered in the culture supernatant protein profile, but is very similar to the wild type in its periplasmic protein profile. R. l. bv. viciae 3841 and the tatC mutant (A1052) were grown for three days in 100 ml TY (periplasm) or for four days in 100 ml Y-mannitol medium (culture supernatant). Periplasmic proteins were prepared by cold hypo-osmotic shock, ~30 μg were loaded per lane. Culture supernatant proteins were precipitated with TCA and protein representing 20 ml of culture were loaded in each lane.

Back to article page