qRT-PCR confirmation of SuperSAGE results. TaqMan™ RT-PCR confirmation of SuperSAGE data by relative curve quantification using primers and TaqMan™ probes designed from 3'- and 5'-RACE sequences, respectively, that are derived from corresponding 26 bp tags. A) TaqMan™ assay with tag primer XPTm-Ca-22356 (derived from a 3'-RACE product; the original tag represents O65741_CICAR, the mRNA for a putative trans-membrane channel protein). Result: no difference between control and desiccated roots, i.e. constitutive expression (black arrow). B) TaqMan™ assay with tag primer XPTm-Ca-7975 (derived from a 3'-RACE product; the original tag represents an anonymous drought-induced EST). Result: earlier CT for the cDNA from desiccated roots (double headed arrow). Control (red), drought-stressed roots (blue).