Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction

BMC Genomics

Table 3 Sensitivity of GM padlock probes.

	Bt176	MON1445	RRS	GM mix
CaMV P35S	0.1%	0.5%	0.1%	1.0%
FMV P35S	n.p.	5.0%	n.p.	1.7%
*bar*	0.1% (0.5*)	n.p.	n.p.	1.7%
Bt176 event	0.5%	n.p.	n.p.	2.5%
RRS event	n.p.	n.p.	5.0%	2.5%

Bt176, MON1445 and RRS performed as simplex, GM mix performed as multiplex.
*level of sensitivity when all 'negative' samples were included in the analysis.
n.p.: not present.
1.0% GM mix consists of 20% maize, soya and cotton (5% GMO each) and 40% canola.
1.7% GM mix consists of 33% maize, soya and cotton (5% GMO each).
2.5% GM mix consists of 50% maize and soya (5% GMO each).

ISSN: 1471-2164