Figure 8From: Transcriptional activity around bacterial cell death reveals molecular biomarkers for cell viabilityTranscription level validation with quantitative PCR. The bars indicate the results of a quantitative PCR (Q-PCR) expressed in equivalents of femto grams (fg) of the B. subtilis chromosomal DNA used for calibration (see Methods). The Q-PCR was carried out for 4 primer-probe combinations, designed for the gene encoding dnaK, gspA, cotJA and 23S rRNA genes, as listed in Table 2. The expression levels in femto grams were normalized to the level of 23S rRNA (see the electroferogram in Figure 2).Back to article page