Table 2 List of primer probe combinations for Q-PCR
From: Transcriptional activity around bacterial cell death reveals molecular biomarkers for cell viability
Name | Sequence | Primer Type | Amplicon size |
|---|---|---|---|
B.sub-gspA | ACAAAAGCCGCGTATTA | TAQMAN MGB | 62 bp |
B.sub-gspA-F | GCGGTTGAGAGCAGCCATA | Forward | |
B.sub gspA-R | ATTAAGTCGGGAATCGAAATGC | Reverse | |
B.sub-dnaK | AAGTTCGTTCAACTGCCGG | TAQMAN MGB | 61 bp |
B.sub-dnaK-F | TGAGCTTGGCGACGGTGTA | Forward | |
B.sub-dnaK-R | CCCACCCAGACGGTTGTC | Reverse | |
B.sub-cotJA | CGATGGAGGCTTTGAG | TAQMAN MGB | 59 bp |
B.sub-cotJA-F | GAGCACATGGAGCAGTTTTCG | Forward | |
B.sub-cotJA-R | TCCTTCCAAAGGGTGCCTTT | Reverse | |
B.sub-cheW | TACCGTACACGAAAGTGAA | TAQMAN MGB | 63 bp |
B.sub-cheW-F | TGGATGAAGCGAATGATGTGA | Forward | |
B.sub-cheW-R | CGCCTTCTGGAGCAGATTCT | Reverse | |
B.sub-23S | CGGTACAGAGTGTCCTAC | TAQMAN MGB | 97 bp |
B.sub-23S-F | GGCGAGCGAAACGGGAT | Forward | |
B.sub 23-S-R | ACCTCTTCATCTACCTCGTTCCTTT | Reverse |