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Table 4 Heat-induced expression levels of cotJA in B. subtilis σ-factor mutants

From: Transcriptional activity around bacterial cell death reveals molecular biomarkers for cell viability

strain

EL30°C (pg)

EL60°C (pg)

2log R

168 1A1 (wt)

0.02

0.50

4.7

TNO2007.164 (sigB)

0.01

0.52

5.6

TNO2007.160 (sigD)

0.03

0.59

4.2

TNO2007.166 (sigF)

0.77

0.05

-3.8

TNO2007.167 (sigE)

0.48

0.06

-2.9

TNO2007.169 (sigK)

0.04

0.77

4.3

  1. Listed in columns is the B. subtilis strain, the cotJA gene expression level at 30°C (EL30°C) expressed in picograms (pg) B. subtilis chromosome equivalents, normalized to the amount of isolated 23S rRNA transcript, the cotJA gene expression level at 60°C (EL60°C) expressed in picograms (pg) B. subtilis chromosome equivalents, normalized to the amount isolated of 23S rRNA transcript, and the 2log ratios of expression levels at 60°C (column 2) and the reference temperature at 30°C (columns 1). All values listed in this table were obtained by quantitative PCR with primer probe combinations listed in Table 2.