Figure 2From: Microarray-based estimation of SNP allele-frequency in pooled DNA using the Langmuir kinetic modelAverage background-corrected signal intensity as a function of allele spotting concentration for the six SNPs. All curves depict the non-linear relationship modeled as Langmuir kinetic function for signal intensity from microarray. The spotting concentration of the allelic sequences ranged from 0 to 50 μM for each SNP. The signal intensity is calculated by integrating line profiles for spots containing the perfect match and normalizing the signals. The solid and dashed lines in each plot show best fit to the Langmuir kinetic model for the two alleles.Back to article page