The RAST Server: Rapid Annotations using Subsystems Technology

BMC Genomics

Table 3 A detailed manual analysis

		missing in RAST		missing in SEED
Genome	RNAs	hypoth.	non-hypo	hypoth.	non-hypo
Alcanivorax borkumensis SK2	51	113	38	49	16
Methanocaldococcus jannaschii DSM 2661	43	105	25	74	19
Wolinella succinogenes DSM 1740	45	40	44	98	22

A detailed manual analysis of the genes called in RAST and in the SEED sheds some light on the differences in the respective predictions. As the matching was performed on protein sequences, RNAs could not be matched. Genes found in the SEED and not predicted in the RAST were split into two categories with and without hypothetical annotations. Additional predictions found in RAST but not in SEED were also included and again split into hypothetical and non-hypothetical.

ISSN: 1471-2164