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Table 3 Comparison between the results from microarray and gel assay*

From: A highly sensitive and specific system for large-scale gene expression profiling

  

Microarray Intensity

Gel Assay Intensity

Ratio ADR/MCF-7

Gene Group

Gene Symbol

ADR

MCF-7

ADR

MCF-7

Micro-array

Gel Assay

I

BAI1

1,192

12,372

4,578

1,320

0.10

3.47

 

SSTR2

9,206

19,108

2,887

7,684

0.48

0.38

 

MCL1

17,027

31,012

7,481

8,901

0.55

0.84

 

TPM2

8,582

14,489

34,703

13,186

0.59

2.63

 

ACOX1

8,125

13,350

26,206

19,508

0.61

1.34

 

MGAT4B

2,274

3,219

1,053

922

0.71

1.14

 

UBQLN1

27,113

38,083

24,037

20,089

0.71

1.20

 

RAB3B

28,188

31,908

33,571

31,044

0.88

1.08

 

DNMT1

25,315

27,675

41,164

43,718

0.91

0.94

 

BUB1B

13,233

14,452

21,197

14,321

0.92

1.48

 

PRDM10

13,233

14,452

9,419

6,641

0.92

1.42

 

NOTCH3

7,187

7,618

26,090

24,176

0.94

1.08

 

YY1

27,020

27,930

40,692

33,876

0.97

1.20

 

TPR

13,081

13,176

32,959

26,423

0.99

1.25

 

PTTG1IP

12,506

12,298

15,621

12,424

1.02

1.26

 

PPP2R4

16,105

15,740

15,593

19,497

1.02

0.80

 

YES1

21,006

20,434

18,228

14,830

1.03

1.23

 

ZNF670

23,207

22,487

6,581

6,904

1.03

0.95

 

RAP1GDS1

16,806

15,385

32,743

22,443

1.09

1.46

 

CKS2

21,507

19,672

10,614

9,289

1.09

1.14

 

HDAC3

26,309

22,572

28,826

29,169

1.17

0.99

 

GAPDH

9,369

7,916

34,584

33,939

1.18

1.02

 

PTTG1

12,802

10,790

18,680

16,447

1.19

1.14

 

HSPA5

19,233

16,146

17,077

15,423

1.19

1.11

 

ACTB

6,849

4,400

35,335

35,859

1.56

0.99

 

ASPH

10,942

4,035

20,194

3,502

2.71

5.77

 

S100A2

3,498

1,057

13,688

9,559

3.31

1.43

 

TFG

13,571

3,915

14,015

15,954

3.47

0.88

 

EGFR

6,841

1,676

34,449

7,738

4.08

4.45

 

RELA

8,209

1,970

20,099

12,783

4.17

1.57

 

VIM

12,172

2,192

48,836

2,518

5.55

19.39

 

RTN1

8,770

1,133

17,159

2,008

7.74

8.55

II

CRABP1

94

8,594

0

21,529

0.01

0.00

 

BMP7

92

8,825

0

43,366

0.01

0.00

 

TIMP3

92

5,428

0

17,135

0.02

0.00

 

AGA

90

3,457

0

20,178

0.03

0.00

 

CXCL12

97

24,456

28

16,471

0.00

0.00

 

B2M

98

10,621

990

13,599

0.01

0.07

 

TYRP1

181

5,380

2,125

7,089

0.03

0.30

 

PDGFB

352

4,080

2,460

6,627

0.09

0.37

 

GATA3

176

2,618

2,780

4,343

0.07

0.64

 

SEPP1

84

7,626

3,056

4,432

0.01

0.69

 

TBX2

202

2,255

4,808

9,482

0.09

0.51

 

HDAC5

243

1,266

16,605

7,834

0.19

2.12

III

ETS1

22,413

280

40,334

0.00

79.97

-

 

SPARC

9,959

266

44,142

0.00

37.45

-

 

DSC3

18,136

304

8,076

0.00

59.62

-

 

CD40

17,887

319

15,499

0.00

56.04

-

 

FLT1

16,379

209

4,032

371

78.23

10.87

 

INA

18,499

438

7,908

411

42.27

19.23

 

CASP1

8,146

298

17,307

447

27.34

38.72

 

CDH2

12,318

200

10,259

477

61.58

21.53

 

GNG11

26,835

213

21,000

576

126.28

36.49

 

MYL9

4,007

302

27,023

1,017

13.26

26.57

 

CITED1

996

253

9,542

2,606

3.93

3.66

 

DAB2

1,568

297

32,458

5,136

5.28

6.32

 

CTSZ

12,657

211

22,322

17,993

59.87

1.24

IV

GNRHR

92

214

27

0.00

0.43

-

 

STK32B

82

208

0.00

241

0.39

0.00

 

IL13

82

191

0.00

464

0.43

0.00

 

CDH5

88

213

0.00

519

0.41

0.00

 

IL3

95

203

86

162

0.47

0.53

 

LYL1

85

218

128

64

0.39

2.01

 

CA1

87

192

365

95

0.45

3.83

 

TYROBP

91

199

487

169

0.46

2.89

 

IL13RA2

87

187

1,771

433

0.47

4.09

 

IRF8

86

212

3,695

571

0.41

6.47

 

MMP11

145

234

3,060

2,308

0.62

1.33

  1. Note: 1. Signal intensities of microarray are averages of those respective non-single cell samples. 2. ADR: NCI/ADR-RES.