Figure 1

A balanced loop hybridization scheme for four treatments with five independent biological replicates. Circles represent treatment samples. Sample code: 0.x = replicate x of solvent control worms (C); 1.x = replicate x of 30 mg RDX/kg soil treated worms (R); 2.x = replicate x of 50 mg TNT/kg soil treated worms (T); 3.x = replicate x of 50 mg TNT and 30 mg RDX/kg soil treated worms (M); x = 1-5. Arrows represent array hybridizations between respective samples where the arrowhead indicates Alexa 647 dye labeling and the base of arrows indicate Cy3 dye labeling. See Additional file Additional file 1: Table S1 for more details of the hybridization scheme.