Evaluation of methods to purify virus-like particles for metagenomic sequencing of intestinal viromes

BMC Genomics

Table 2 Intra- and between method variation based on read abundance ratios*

	Intra-method variation				Between method variation ^a
	FD1/FD2	DTT1/DTT2	CsCl1/CsCl2	MG1/MG2	FD/DTT	DTT/CsCl	CsCl/FD
Calculated with % read abundance
M13	0.74	2.22	1.67	1.08	1.52	10.06	0.07
P22	0.96	1.01	1.01	1.73	0.95	0.78	1.36
ɸVPE25	0.94	0.92	0.75	2.33	0.99	125.23	0.01
T3	1.01	1.01	1.14	1.69	0.93	0.94	1.15
T7	1.03	1.00	0.71	1.88	0.94	1.57	0.68
Calculated with % read abundance normalized to genome size
M13	0.75	2.24	1.63	0.73	1.60	11.20	0.06
P22	0.98	1.02	1.01	1.19	1.00	0.91	1.10
ɸVPE25	0.96	0.93	0.75	1.60	1.05	146.16	0.01
T3	1.03	1.01	1.14	1.16	0.98	1.09	0.93
T7	1.05	1.00	0.71	1.29	0.99	1.83	0.55

*Perfect reproducibility would result in ratios equal to one.
^a:Read abundance ratios between methods were calculated using the average read abundance in each method.

ISSN: 1471-2164