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Table 3 Purification method biases for specific phages*

From: Evaluation of methods to purify virus-like particles for metagenomic sequencing of intestinal viromes

 

FD

DTT

CsCl

MG

Average % read abundance normalized to genome size

M13

0.010

0.007

0.001

0.027

P22

79.605

79.695

87.830

29.338

ɸVPE25

5.564

5.310

0.036

9.749

T3

6.164

6.286

5.751

2.101

T7

4.879

4.923

2.691

2.974

Ratios of genome size normalized % read abundances

P22/ɸVPE25

14.3

15.0

2417.5

3.0

P22/T3

12.9

12.7

15.3

14.0

P22/T7

16.3

16.2

32.6

9.9

ɸVPE25/T3

0.9

0.8

0.0

4.6

ɸVPE25/T7

1.1

1.1

0.0

3.3

T3/T7

1.3

1.3

2.1

0.7

T7/M13

465

748

4584

108

P22/M13

7587

12119

149630

1073

ɸVPE25/M13

530

807

61

356

T3/M13

587

956

9797

76

  1. *In case of absence of purification biases, ratios of size normalized read abundances between phages would be identical in the unpurified control samples (MG) and the purified samples (FD, DTT and CsCl).